Cloning and characterization of elastase genes from Pseudomonas aeruginosa.

  title={Cloning and characterization of elastase genes from Pseudomonas aeruginosa.},
  author={P. A. Schad and R A Bever and Thalia I. Nicas and Fr{\'e}d{\'e}ric Leduc and Larry F Hanne and Barbara H. Iglewski},
  journal={Journal of bacteriology},
  volume={169 6},
A gene bank was constructed from Pseudomonas aeruginosa PAO1 and used to complement three P. aeruginosa elastase-deficient strains. One clone, pRF1, contained a gene which restored elastase production in two P. aeruginosa isolates deficient in elastase production (PA-E15 and PAO-E105). This gene also encoded production of elastase antigen and activity in Escherichia coli and is the structural gene for Pseudomonas elastase. A second clone, pHN13, contained a 20-kilobase (kb) EcoRI insert which… CONTINUE READING


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