Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid

@article{Brown1993CloningAC,
  title={Cloning and characterization of an extracellular Ca2+-sensing receptor from bovine parathyroid},
  author={Edward Meigs Brown and Gerardo Gamba and Daniela Riccardi and Michael Lombardi and Robert R Butters and Olga Kifor and Adam M Sun and Matthias A. Hediger and Jonathan Lytton and Steven C. Hebert},
  journal={Nature},
  year={1993},
  volume={366},
  pages={575-580}
}
MAINTENANCE of a stable internal environment within complex organisms requires specialized cells that sense changes in the extracellular concentration of specific ions (such as Ca2+). Although the molecular nature of such ion sensors is unknown, parathyroid cells possess a cell surface Ca2+-sensing mechanism that also rec-ognizes trivalent and polyvalent cations (such as neomycin) and couples by changes in phosphoinositide turnover and cytosolic Ca2+ to regulation of parathyroid hormone… Expand
Cloning and functional expression of a rat kidney extracellular calcium/polyvalent cation-sensing receptor.
TLDR
In rat kidney, Northern analysis revealed RaKCaR transcripts of 4 and 7 kb, and in situ hybridization showed localization primarily in outer medulla and cortical medullary rays, which provide important insights into the molecular structure of an extracellular Ca2+/polyvalent cation-sensing receptor in rat kidney. Expand
Sensing of extracellular Ca2+ by parathyroid and kidney cells: cloning and characterization of an extracellular Ca(2+)-sensing receptor.
  • E. Brown, M. Pollak, S. Hebert
  • Biology, Medicine
  • American journal of kidney diseases : the official journal of the National Kidney Foundation
  • 1995
TLDR
The hypercalcemic disorders, familial hypocalciuric hypercalcemia and neonatal severe hyperparathyroidism are investigated, since both disorders show abnormal Ca2+ o-sensing and/or handling in the kidney and parathyroid. Expand
Cloning and functional characterization of extracellular Ca(2+)-sensing receptors from parathyroid and kidney.
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The availability of the cDNA clone for the Ca2+o-sensing receptor made it possible to test the hypothesis that mutations in the gene encoding the human homolog of the receptor cause inherited disorders of mineral ion metabolism, and discovered a family in which a form of autosomal dominant hypocalcemia results from an activating mutation in the receptor gene. Expand
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This Ca;; -sensing receptor permits Ca„ ' to act as an extracellular, first messenger in addition to its better known role as an intracellular second messenger. Expand
Molecular Cloning and Functional Expression of Human Parathyroid Calcium Receptor cDNAs (*)
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While there is no evidence for alteration of the primary amino acid sequence of the CaR in this adenoma, modulation of CaR biosynthesis through alternative RNA processing may play a role in set-point alterations. Expand
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Functional evidence for the participation of CaRG in the regulation of renal Ca2+ reabsorption in TAL and water re absorption in the collecting duct has been obtained and speculation on the potential involvement in other pathologies is allowed. Expand
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  • Biology, Medicine
  • Journal of the American Society of Nephrology : JASN
  • 1995
TLDR
This cDNA encoding a Ca2+o-sensing receptor from bovine parathyroid and rat kidney permits extracellular calcium ions to act not only as an intracellular second messenger but also in a "hormone-like" role as anextracellular first messenger. Expand
Extracellular calcium sensing and extracellular calcium signaling.
The cloning of a G protein-coupled extracellular Ca(2+) (Ca(o)(2+))-sensing receptor (CaR) has elucidated the molecular basis for many of the previously recognized effects of Ca(o)(2+) on tissuesExpand
Identification of a Novel Extracellular Cation-sensing G-protein-coupled Receptor*
The C family G-protein-coupled receptors contain members that sense amino acid and extracellular cations, of which calcium-sensing receptor (CASR) is the prototypic extracellular calcium-sensingExpand
G protein-coupled, extracellular Ca2+ (Cao2+)-sensing receptor enables Cao2+ to function as a versatile extracellular first messenger
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  • Biology, Medicine
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  • 2007
The cloning of a G protein-coupled, extracellular Ca2+ (Cao2+)-sensing receptor (CaR) has afforded a molecular basis for a number of the known effects of Cao2+ on tissues involved in maintainingExpand
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