Cloning and characterization of an autonomous replication sequence from Coxiella burnetii.

@article{Suhan1994CloningAC,
  title={Cloning and characterization of an autonomous replication sequence from Coxiella burnetii.},
  author={M L Suhan and Stacy Y. Chen and Herbert A. Thompson and Timothy A. Hoover and Alyssa Hill and Jim C. Williams},
  journal={Journal of bacteriology},
  year={1994},
  volume={176 17},
  pages={5233-43}
}
A Coxiella burnetii chromosomal fragment capable of functioning as an origin for the replication of a kanamycin resistance (Kanr) plasmid was isolated by use of origin search methods utilizing an Escherichia coli host. The 5.8-kb fragment was subcloned into phagemid vectors and was deleted progressively by an exonuclease III-S1 technique. Plasmids containing progressively shorter DNA fragments were then tested for their capability to support replication by transformation of an E. coli polA… CONTINUE READING
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