Cloning and characterization of an Onchocerca volvulus specific DNA sequence.

  title={Cloning and characterization of an Onchocerca volvulus specific DNA sequence.},
  author={S. E. O. Meredith and Thomas R. Unnasch and Matthew Karam and Willy F. Piessens and Dyann F Wirth},
  journal={Molecular and biochemical parasitology},
  volume={36 1},
A cloned sequence, pOvs134, was isolated from a genomic library prepared from Onchocerca volvulus of savanna origin in the plasmid pUC9. pOvs134 hybridizes to all the geographic isolates of O. volvulus tested from both the New and the Old World, but not to the species Onchocerca gibsoni, Onchocerca gutturosa, Onchocerca ochengi, Onchocerca cervicalis, the filarial parasites Brugia malayi, or Dirofilaria immitis, nor to human or simuliid DNA. As little as 250 pg of DNA can be detected on a dot… CONTINUE READING

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