Cloning and characterization of an Mn-containing superoxide dismutase (SodA) of Bordetella pertussis.

@article{GraeffWohlleben1997CloningAC,
  title={Cloning and characterization of an Mn-containing superoxide dismutase (SodA) of Bordetella pertussis.},
  author={Heinke Graeff-Wohlleben and S Killat and A Banemann and Nicole Guiso and Roland Gross},
  journal={Journal of bacteriology},
  year={1997},
  volume={179 7},
  pages={2194-201}
}
The Fur titration assay (FURTA) recently developed by I. Stojiljkovic and coworkers (J. Mol. Biol. 236:531-545, 1994) was applied to clone iron-regulated genes of Bordetella pertussis. After sequence analysis, one of the clones obtained by this selection procedure was shown to contain an open reading frame with significant sequence similarities to Mn-containing superoxide dismutases (SodA). The open reading frame was preceded by a Fur consensus binding site, which according to primer extension… CONTINUE READING
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