Cloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch

@article{Yang2004CloningAC,
  title={Cloning and characterization of an IGF-1 isoform expressed in skeletal muscle subjected to stretch},
  author={Shiyu Yang and M. Alnaqeeb and H. Simpson and G. Goldspink},
  journal={Journal of Muscle Research \& Cell Motility},
  year={2004},
  volume={17},
  pages={487-495}
}
SummaryTo ascertain if IGF-1 is a regulator of local muscle growth, total RNA was extracted from rabbit muscle induced to undergo rapid hypertrophy using active stretch and from control muscles. This was analysed by Northern hybridization with a 280 base pair probe containing sequences derived from exons 3 and 4 of the insulin-like growth factor 1 gene. Two types of insulin-like growth factor 1 mRNA were shown to be strong expressed in the stretched muscles. In situ hybridization using the same… Expand
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References

SHOWING 1-10 OF 43 REFERENCES
Activation of insulin-like growth factor gene expression during work-induced skeletal muscle growth.
TLDR
Results indicate that signals propagated during muscle hypertrophy enhance the expression of both IGF genes, that modulation of IGF-I mRNA levels can occur in the absence of growth hormone, and that locally produced IGF- I and IGF-II may play a role in skeletal muscle growth. Expand
Mosaic evolution of the insulin-like growth factors. Organization, sequence, and expression of the rat insulin-like growth factor I gene.
TLDR
A comparably high degree of amino acid sequence identity is found for both the amino- and carboxyl-terminal extension peptides, suggesting that, like mature IGF-I, the extension molecules may have physiological function. Expand
The porcine insulin-like growth factor-I gene: characterization and expression of alternate transcription sites.
TLDR
The expression of class 1 and 2 transcripts was measured in liver and muscle RNA from two groups of 2-month-old pigs whose energy status had been manipulated within physiological limits to produce marked differences in plasma IGF-I levels and growth rates, suggesting that liver-derived endocrine IGF-i has an important function in the regulation of muscle growth and that class 2 IGF- I transcripts are more sensitive to conditions that promote optimal growth. Expand
Induction of insulin-like growth factor I messenger ribonucleic acid during regeneration of rat skeletal muscle.
TLDR
In situ hybridization at the time of maximum induction showed the presence of IGF-I mRNA in proliferating myoblasts and in satellite cells, suggesting IGF-i may act as a locally produced non-GH dependent trophic factor during regeneration of skeletal muscle after injury. Expand
Regulation of insulin-like growth factor 1 gene expression in skeletal muscle.
TLDR
In contrast insulin dramatically affected muscle protein synthesis rates but had no measurable effect upon muscle IGF-1 mRNA levels, which suggests that the anabolic action of this hormone is not mediated through the autocrine/paracrine action of IGF- 1. Expand
The ovine insulin-like growth factor-I gene: characterization, expression and identification of a putative promoter.
TLDR
expression of IGF-I mRNA, as measured by a solution hybridization/RNase protection assay, predominated in the liver of the neonate and the late-gestation fetus; however, lower levels of expression were seen in multiple tissues throughout fetal and neonatal development. Expand
Sequence of a cDNA clone encoding human preproinsulin-like growth factor II
TLDR
As a first step in studying the biosynthesis of these proteins and elucidating their role(s) in normal development and in tumorigenesis, isolated and sequenced cDNAs prepared from adult human liver mRNA which encode the precursors to IGF-I and -II are reported. Expand
Molecular cloning of rat insulin-like growth factor I complementary deoxyribonucleic acids: differential messenger ribonucleic acid processing and regulation by growth hormone in extrahepatic tissues.
TLDR
Two classes of insulin-like growth factor I (IGF-I) cDNAs were isolated from an adult rat liver library using a human IGF-I cDNA probe and hybridization to poly(A)+ RNA from various tissues from GH-treated and control rats demonstrated an increase in IGF- I mRNA due to GH treatment in all tissues examined. Expand
Satellite cells express the trophic factor IGF-I in regenerating skeletal muscle.
TLDR
It is concluded that IGF-I may act as a trophic factor during regeneration of skeletal muscle after injury while RR immunoreactivity was seen only in scattered satellite cells. Expand
Gene expression in skeletal muscle in response to stretch and force generation.
TLDR
RNA analyses after cast immobilization of the limb with the muscle in the lengthened or shortened position and/or with electrical stimulation were used to determine the effects of altered mechanical signals on gene transcription. Expand
...
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2
3
4
5
...