Cloning and characterization of a transcription factor that binds to the proximal promoters of the two mouse type I collagen genes.

Abstract

We have used the yeast one-hybrid system to clone transcription factors that bind to specific sequences in the proximal promoters of the type I collagen genes. We utilized as bait the sequence between -180 and -136 in the pro-alpha2(I) collagen promoter because it acts as a functional promoter element and binds several DNA-binding proteins. Three cDNA… (More)

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