Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco cells.

@article{Taguchi2003CloningAC,
  title={Cloning and characterization of a glucosyltransferase that reacts on 7-hydroxyl group of flavonol and 3-hydroxyl group of coumarin from tobacco cells.},
  author={Goro Taguchi and Takahisa Ubukata and Nobuaki Hayashida and Hirobumi Yamamoto and Mitsuo Okazaki},
  journal={Archives of biochemistry and biophysics},
  year={2003},
  volume={420 1},
  pages={95-102}
}
In higher plants, secondary metabolites are often converted to their glycoconjugates by glycosyltransferases (GTases). We cloned a cDNA encoding GTase (NtGT2) from tobacco (Nicotiana tabacum L.). The recombinant enzyme expressed in Escherichia coli (rNTGT2) showed glucosylation activity against several kinds of phenolic compounds, particularly the 7-hydroxyl group of flavonoids and 3-hydroxycoumarin. The K(m) values of kaempferol and 3-hydroxycoumarin with rNTGT2 are 6.5 microM and 23.6 microM… CONTINUE READING

From This Paper

Figures, tables, results, connections, and topics extracted from this paper.
13 Extracted Citations
0 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 13 extracted citations

Similar Papers

Loading similar papers…