Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454).

@article{Sekiguchi2005CloningAC,
  title={Cloning and characterization of a novel trimethoprim-resistant dihydrofolate reductase from a nosocomial isolate of Staphylococcus aureus CM.S2 (IMCJ1454).},
  author={Jun-ichiro Sekiguchi and Prasit Tharavichitkul and Tohru Miyoshi-Akiyama and Vena Chupia and Tomoko Fujino and Minako Araake and Atsushi Irie and Koji Morita and Tadatoshi Kuratsuji and Teruo Kirikae},
  journal={Antimicrobial agents and chemotherapy},
  year={2005},
  volume={49 9},
  pages={3948-51}
}
A novel gene, dfrG, encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP. 

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