Cloning and characterization of a novel lipase from Vibrio harveyi strain AP6.

@article{Teo2003CloningAC,
  title={Cloning and characterization of a novel lipase from Vibrio harveyi strain AP6.},
  author={Jeanette W P Teo and L Zhang and Chit Laa Poh},
  journal={Gene},
  year={2003},
  volume={312},
  pages={181-8}
}
A lipolytic enzyme designated Vst, was cloned from Vibrio harveyi strain AP6. The vst gene was 1650 bp and was predicted to encode a 549 amino acid precursor with a molecular mass of about 61 kDa. The predicted polypeptide shared about 30% sequence identity with Bacillus esterases. Sequence alignment of Vst and related esterases revealed the presence of an active site serine located in the middle of the GESAG motif, at positions 212-216. This motif resembled the GXSXG consensus motif… CONTINUE READING