Cloning and characterization of a novel chromosomal drug efflux gene in Staphylococcus aureus.

@article{Narui2002CloningAC,
  title={Cloning and characterization of a novel chromosomal drug efflux gene in Staphylococcus aureus.},
  author={Koji Narui and Norihisa Noguchi and Kazunori Osuke Wakasugi and Masanori Sasatsu},
  journal={Biological & pharmaceutical bulletin},
  year={2002},
  volume={25 12},
  pages={1533-6}
}
A novel drug efflux gene (named sepA, staphylococcal efflux pump gene) is cloned from antiseptic-resistant mutants of Staphylococcus aureus into Escherichia coli. The sepA gene conferred the reduction of susceptibility to acriflavine and the acceleration of ethidium bromide efflux from the E. coli cells. The sepA (474 bp) encoded the protein that has four predicted transmembrane segments. These results indicate that sepA gene is a multidrug-resistant gene and encodes a drug efflux protein. 
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Growth of S. aureus RN2677 Carrying Various Plasmids on a Gradient Plate The minimum and maximum acriflavine concentrations (mg/ml) on the gradient plate are shown

  • N. Noguchi, A. Emura, M. Sasatsu, M. Kono, Bull. Biol. Pharm
  • 1994

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