Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome

@article{Jeon2008CloningAC,
  title={Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome},
  author={Jeong Ho Jeon and Jun-Tae Kim and Yun Jae Kim and Hyung-Kwoun Kim and Hyun Sook Lee and Sung Gyun Kang and Sang-Jin Kim and Jung-Hyun Lee},
  journal={Applied Microbiology and Biotechnology},
  year={2008},
  volume={81},
  pages={865-874}
}
To search for new cold-active lipases, a metagenomic library was constructed using cold-sea sediment samples at Edison Seamount and was screened for lipolytic activities by plating on a tricaprylin medium. Subsequently, a fosmid clone was selected, and the whole sequence of 36 kb insert of the fosmid clone was determined by shotgun sequencing. The sequence analysis revealed the presence of 25 open reading frames (ORF), and ORF20 (EML1) showed similarities to lipases. Phylogenetic analysis of… CONTINUE READING