Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases

  title={Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases},
  author={Masaki Okano and Shaoping Xie and En Li},
  journal={Nature Genetics},
Cloning and characterization of a family of novel mammalian DNA (cytosine-5) methyltransferases 
DNA Methyltransferases and Structural-Functional Specificity of Eukaryotic DNA Modification
Structural-functional specificity of eukaryotic genome sequences methylated by DNA methyltransferases is characterized and the total methylation of cytosine in DNA sequences is described, as well as its relation with RNA interference.
Role of de π0卯 DNA Methyltransferases Dn肌t3α and Dπm岱h 血 the Establishment of Genomic lmprinting
Introduction Materials and Methods 入lice Isolation of DNA and RNA : The construction of a probabilistic procedure for the isolation of RNA from DNA and its structure is described.
Cytosine Methylation: Remaining Faithful
DNA Modifications: Methods and Protocols
An overview of the spectrum of cytosine modifications and their roles in demethylation of DNA and regulation of gene expression in different eukaryotic organisms is provided.
Diverse Domains of (Cytosine-5)-DNA Methyltransferases: Structural and Functional Characterization
(Cytosine-5)-DNA methyltransferases (C5-DNA MTases) are enzymes which catalyze meth‐ yl group transfer from S-adenosyl-L-methionine (AdoMet) to C5 atom of cytosine residue in DNA. As a result, AdoMet
Mammalian cytosine methylation at a glance
In mammals, methylation at symmetric CpG dinucleotides is necessary for the heritable silencing and regulation of many different classes of DNA sequences. These include highly abundant
Mapping and elucidating the function of modified bases in DNA
The Balasubramanian laboratory is supported by core funding from Cancer Research UK (C14303/A17197) and the Wellcome Trust (Grant No. 099232/z/12/z).
Leukemia stem cell fates are determined by DNA methylation levels
Zur Erlangung des akademischen Grades d o c t o r r r e r u m n a t u r a l i u m ist es wirklich nur ein wirklicher Schritt für die ganze Wirklichkeit.
The Human Dnmt2 Has Residual DNA-(Cytosine-C5) Methyltransferase Activity*
It is shown by two independent assay systems that the purified Dnmt2 protein has weak DNA methyltransferase activity, and methylation was observed at CG sites in a loose ttnCGga(g/a) consensus sequence, suggesting that DnMT2 has a more specialized role than other mammalian DNA methyl transferases.


DNA Methylation in Early Mammalian Development
The genomic DNAs of vertebrates and many invertebrates contain 5-methylcytosine (5-meCyt) as the only modified base and an inverse correlation between methylation and transcriptional activity has been found for a number of developmentally regulated genes.
The DNA (cytosine-5) methyltransferases
The reaction pathway has suddenly become more complicated because of the base-flipping and much remains to be learned about the DNA recognition elements in the family members for which structural information is not yet available.
A candidate mammalian DNA methyltransferase related to pmt1p of fission yeast.
Dnmt2 is a candidate for the activity that methylates newly integrated retroviral DNA and maintains trace levels of 5-methylcytosine in the DNA of embryonic stem cells homozygous for null mutations in Dnmt1, the gene for the one previously recognized metazoan DNA methyltransferase.
Baculovirus-mediated expression and characterization of the full-length murine DNA methyltransferase.
The homogeneous recombinant enzyme from the fourth ATG construct shows a 2.5-fold preference for a hemi-methylated DNA substrate as compared to an unmethylated substrate, whereas the 185 kDa protein is equally active on both substrates.
DNA methylation and embryogenesis.
A large number of studies have documented the methylation patterns of individual genes and repeated sequences in different tissues of various mammalian species. This has revealed a consistent picture
Dnmt2 is not required for de novo and maintenance methylation of viral DNA in embryonic stem cells.
It is shown that endogenous virus was fully methylated in Dnmt2 -deficient mutant ES cells and newly integrated retrovirus DNA was methylated de novo in infected mutantES cells as efficiently as in wild-type cells.
DNA methylation, biochemistry, and biological significance
This chapter discusses DNA Methylation and Developmental Regulation of Eukaryotic Globin Gene Transcription, and left-handed Z-DNA and M methylation of d(CpG) Sequences.
Altered DNA methylation and genome instability: a new pathway to cancer?
  • P. Jones, M. Gonzalgo
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1997
There is growing evidence that methylation plays a pivotal role in key developmental processes such as genomic imprinting and stabilization of X-chromosome inactivation, and it therefore is not surprising that alterations in this essential epigenetic system might play a role in carcinogenesis.