Cloning and characterization of a cDNA from Aspergillus parasiticus encoding an O-methyltransferase involved in aflatoxin biosynthesis.

@article{Yu1993CloningAC,
  title={Cloning and characterization of a cDNA from Aspergillus parasiticus encoding an O-methyltransferase involved in aflatoxin biosynthesis.},
  author={Jiujiang Yu and Jeffrey W. Cary and Deepak Bhatnagar and Thomas E. Cleveland and Nancy P Keller and Fun Sun Chu},
  journal={Applied and environmental microbiology},
  year={1993},
  volume={59 11},
  pages={3564-71}
}
Aflatoxins are polyketide-derived secondary metabolites produced by the fungi Aspergillus flavus and Aspergillus parasiticus. Among the catalytic steps in the aflatoxin biosynthetic pathway, the conversion of sterigmatocystin to O-methylsterigmatocystin and the conversion of dihydrosterigmatocystin to dihydro-O-methylsterigmatocystin are catalyzed by an S-adenosylmethionine-dependent O-methyltransferase. A cDNA library was constructed by using RNA isolated from a 24-h-old culture of wild-type A… CONTINUE READING

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