Cloning and characterization of a cDNA encoding beta-amyrin synthase involved in glycyrrhizin and soyasaponin biosyntheses in licorice.

@article{Hayashi2001CloningAC,
  title={Cloning and characterization of a cDNA encoding beta-amyrin synthase involved in glycyrrhizin and soyasaponin biosyntheses in licorice.},
  author={Hiroaki Hayashi and Pengyu Huang and Ara Kirakosyan and Kenichiro Inoue and Nobutsugu Hiraoka and Yasumasa Ikeshiro and Tetsuo Kushiro and Masaaki Shibuya and Yutaka Ebizuka},
  journal={Biological & pharmaceutical bulletin},
  year={2001},
  volume={24 8},
  pages={912-6}
}
An oxidosqualene cyclase cDNA, termed GgbAS1, was isolated from cultured cells of licorice (Glycyrrhiza glabra) by heterologous hybridization with cDNA of Arabidopsis thaliana LUP1 lupeol synthase. The yeast transformed with an expression vector containing the open reading frame of GgbAS1 produced beta-amyrin, indicating that GgbAS1 encodes beta-amyrin synthase involved in the glycyrrhizin and soyasaponin biosyntheses in licorice. Northern blot analysis showed that the level of beta-amyrin… CONTINUE READING