Cloning and characterization of a GPAT-like gene from the microalga Lobosphaera incisa (Trebouxiophyceae): overexpression in Chlamydomonas reinhardtii enhances TAG production

@article{Iskandarov2015CloningAC,
  title={Cloning and characterization of a GPAT-like gene from the microalga Lobosphaera incisa (Trebouxiophyceae): overexpression in Chlamydomonas reinhardtii enhances TAG production},
  author={Umidjon Iskandarov and Siarhei Sitnik and Nastassia Shtaida and Shoshana Didi-Cohen and Stefan Leu and Inna Khozin-Goldberg and Z. Cohen and Sammy Boussiba},
  journal={Journal of Applied Phycology},
  year={2015},
  volume={28},
  pages={907-919}
}
The oleaginous green microalga Lobosphaera (formerly Parietochloris) incisa accumulates high amounts of arachidonic-acid-rich triacylglycerols (TAG), in particular under conditions of nitrogen starvation. This photosynthetic organism is of great interest for studying the mechanisms responsible for storage lipid biosynthesis and the deposition of long-chain polyunsaturated fatty acids (LC-PUFA) in TAG. In this work, we report on cloning a complementary DNA (cDNA) for the putative L. incisa… CONTINUE READING
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