Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro.

@article{Zhao2007CloningAC,
  title={Cloning and characterization of GDP-perosamine synthetase (Per) from Escherichia coli O157:H7 and synthesis of GDP-perosamine in vitro.},
  author={Guohui Zhao and Jun Liu and Xiang Liu and Min Chen and Hou-cheng Zhang and Peng George Wang},
  journal={Biochemical and biophysical research communications},
  year={2007},
  volume={363 3},
  pages={
          525-30
        }
}
  • Guohui Zhao, Jun Liu, P. Wang
  • Published 23 November 2007
  • Biology, Chemistry
  • Biochemical and biophysical research communications
Accommodation of GDP-linked sugars in the active site of GDP-perosamine synthase.
TLDR
Light is shed on why GDP-perosamine synthase functions as an aminotransferase whereas another very similar PLP-dependent enzyme, GDP-4-keto-6-deoxy- d-mannose 3-dehydratase or ColD, catalyzes a dehydration reaction using the same substrate.
GDP-perosamine synthase: structural analysis and production of a novel trideoxysugar.
TLDR
A novel GDP-linked sugar is produced by purifying the ColD product and reacting it with purified GDP-perosamine synthase, and the enzymatic synthesis of GDP-4-amino-3,4,6-trideoxy- d-mannose is produced.
Catalytic mechanism of perosamine N-acetyltransferase revealed by high-resolution X-ray crystallographic studies and kinetic analyses.
TLDR
It is shown that PerB can accept GDP-3-deoxyperosamine as an alternative substrate, thus representing the production of a novel trideoxysugar.
O-antigen polymerase adopts a distributive mechanism for lipopolysaccharide biosynthesis
TLDR
This work presents the first biochemical evidence that Wzy functions in a distributive manner, and chemo-enzymatically synthesized the substrates of Wzy, the lipid-PP-linked repeat units.
Characterization of the dehydratase WcbK and the reductase WcaG involved in GDP-6-deoxy-manno-heptose biosynthesis in Campylobacter jejuni.
TLDR
Capillary electrophoresis and MS analyses showed that WcbK is a GDP-manno-heptose dehydratase whose product can be reduced by WcaG, and that WCBK/WcaG can use the substrate GDP-mannose, although with lower efficiency than heptose.
Structural Studies of Lipopolysaccharide-defective Mutants from Brucella melitensis Identify a Core Oligosaccharide Critical in Virulence*
TLDR
The structures of the lipooligosaccharides from Brucella melitensis mutants affected in the WbkD and ManBcore proteins have been fully characterized using NMR spectroscopy and it is reported that the core deficiency in the wadC mutant structure resulted in a more efficient detection by innate immunity and attenuation.
Pathways for the Biosynthesis of NDP Sugars
Bacterial lipopolysaccharide (LPS) is an important surface structure of Gramnegative bacteria for maintaining the integrity of the outer membrane. It is also a virulence factor in many bacteria,
Structural diversity in Salmonella O antigens and its genetic basis.
TLDR
The structures and gene clusters of the GlcNAc/GalNAc-initiated O antigens of Salmonella were found to be highly diverse, and 24 of them were find to be identical or closely related to Escherichia coli O antIGens.
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