Cloning and characterization of 2S albumin, Car i 1, a major allergen in pecan.

@article{Sharma2011CloningAC,
  title={Cloning and characterization of 2S albumin, Car i 1, a major allergen in pecan.},
  author={Girdhari M. Sharma and Andr{\'e} Southernman Teixeira Irsigler and Pushparani Dhanarajan and Rose M Perez Ayuso and Luda Bardina and Hugh A Sampson and Kenneth H. Roux and Shridhar K Sathe},
  journal={Journal of agricultural and food chemistry},
  year={2011},
  volume={59 8},
  pages={
          4130-9
        }
}
Although pecans are associated with IgE-mediated food allergies, the allergens responsible remain to be identified and characterized. The 2S albumin gene was amplified from the pecan cDNA library. Dot-blots were used to screen the recombinant protein with pecan allergic patients' serum. The affinity purified native protein was analyzed by Edman sequencing and mass spectrometry/mass spectrometry (MS/MS) analysis. Cross-reactivity with walnut was determined by inhibition enzyme-linked… CONTINUE READING
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