Cloning and characterisation of the Schizosaccharomyces pombe rad8 gene, a member of the SNF2 helicase family.

@article{Doe1993CloningAC,
  title={Cloning and characterisation of the Schizosaccharomyces pombe rad8 gene, a member of the SNF2 helicase family.},
  author={Claudette L. Doe and J. M. Murray and M. Shayeghi and Marilyn Hoskins and A. R. Lehmann and Antony M Carr and Felicity Z. Watts},
  journal={Nucleic acids research},
  year={1993},
  volume={21 25},
  pages={5964-71}
}
The Schizosaccharomyces pombe rad8 mutant is sensitive to both UV and gamma irradiation. We have cloned the rad8 gene by complementation of the UV sensitivity of a rad8.190 mutant strain. The gene comprises an open reading frame of 3.4 kb which does not contain any introns and is capable of encoding a 1133 amino acid protein of 129 kDa. Deletion of the gene indicates that it is not essential for cell viability. Recognisable motifs are present for a nuclear localisation signal, a RING finger and… CONTINUE READING
7 Citations
2 References
Similar Papers

References

Publications referenced by this paper.
Showing 1-2 of 2 references

http://narrdjournals.org/ D ow nladed fom

  • C Troelstra, A. van Gool, J. de Wit, W. Vermeulen, D. Bootsma, J.H.J. Hoeijmakers
  • U nirsity of S usex on Jne 18,
  • 1992
Highly Influential
12 Excerpts

Molecular cloning, a laboratory manual. Cold Spring Harbor Laborator y Press, Cold Spring Harbor

  • Sambrook.J, E. F. Fritsch, T. E. Maniatis
  • 1989

Similar Papers

Loading similar papers…