Cloning and characterisation of the human 5‐HT5A serotonin receptor

  title={Cloning and characterisation of the human 5‐HT5A serotonin receptor},
  author={Steven Rees and Izaak den Daas and Steven M. Foord and Susannah J. Goodson and D. R. Bull and Gavin Kilpatrick and M G Lee},
  journal={FEBS Letters},
5-ht5A receptors as a therapeutic target.
Molecular biology and potential functional role of 5-HT5, 5-HT6, and 5-HT7 receptors
This review focuses on the structural features, binding properties, and mRNA distribution of these novel 5-HT receptor subtypes, as well as an evaluation of the tool sets available to link these cloned receptors to function and therapeutic potential.
The Serotonin Receptor Subtype 5b Specifically Interacts with Serotonin Receptor Subtype 1A
It is found that 5-ht5b receptors can specifically interact with 5-HT1A receptors and retain them in endosomal compartments, which possibly represents a mechanism by which 4 and fifth trans-membrane domain (TMD) regulates the activity of other 5- HT receptor.
receptor was lost during mammalian evolution
The results suggest, 5B therefore, that the 5-HT receptor has been lost during evolution after the divergence between rodents and primates.
New players in the 5-HT receptor field: genes and knockouts.


The mouse 5HT5 receptor reveals a remarkable heterogeneity within the 5HT1D receptor family.
The results demonstrate that the 5HT1D receptors constitute a heterogeneous family of receptors with distinct intracellular signalling properties and expression patterns, and are a distant relative of all previously identified 5‐HT receptors.
Isolation of a mouse "5HT1E-like" serotonin receptor expressed predominantly in hippocampus.
The results demonstrate that the 5HT1E receptors constitute a heterogeneous family of receptors, similar to other 5-hydroxytryptamine receptors found in human and rat brain.
Two members of a distinct subfamily of 5-hydroxytryptamine receptors differentially expressed in rat brain.
Two serotonin (5-hydroxytryptamine, 5-HT) receptors, MR22 and REC17, that belong to the G-protein-associated receptor superfamily are reported and it is proposed that REC17 and MR22 represent a distinct subfamily of 5- HT receptors.
International Union of Pharmacology classification of receptors for 5-hydroxytryptamine (Serotonin).
It is evident that in the last decade or so, a vast amount of new information has become available concerning the various 5-HT receptor types and their characteristics, and it is important to rationalise in concert all of the available data from studies involving both operational approaches of the classical pharmacological type and those from molecular and cellular biology.
Muscarinic receptor subtypes.
The aim of this review is to discuss the structure, function, and binding properties of the different muscarinic receptor species, attempting where possible to coordinate the diverse experimental data into a uniform picture.
Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure.
  • B. Seed, A. Aruffo
  • Biology, Medicine
    Proceedings of the National Academy of Sciences of the United States of America
  • 1987
A cDNA encoding the CD2 antigen has been isolated by a highly efficient technique based on transient expression in COS cells and adherence of cells expressing surface antigen to antibody-coated
Intervening sequences increase efficiency of RNA 3' processing and accumulation of cytoplasmic RNA.
Two expression vectors were constructed that differ only in the presence of an intervening sequence (IVS) in their 5'-untranslated leaders, and results imply that splicing is coupled to a polyadenylation/transport pathway.
Modified T7 DNA polymerase for DNA sequencing.
Rees et a
  • IFEBS Letters
  • 1994