Cloning and characterisation of the Equilibrative Nucleoside Transporter family of Trypanosoma cruzi: ultra-high affinity and selectivity to survive in the intracellular niche.

@article{Campagnaro2018CloningAC,
  title={Cloning and characterisation of the Equilibrative Nucleoside Transporter family of Trypanosoma cruzi: ultra-high affinity and selectivity to survive in the intracellular niche.},
  author={Gustavo Daniel Campagnaro and Janaina de Freitas Nascimento and Richard M. B. M. Girard and Ariel Mariano Silber and Harry P de Koning},
  journal={Biochimica et biophysica acta. General subjects},
  year={2018},
  volume={1862 12},
  pages={
          2750-2763
        }
}
BACKGROUND Trypanosoma cruzi, the causative agent of Chagas' disease is unable to synthesise its own purines and relies on salvage from the host. In other protozoa, purine uptake has been shown to be mediated by Equilibrative Nucleoside Transporters (ENTs). METHODS To investigate the functionality of T. cruzi-encoded ENT transporters, its four putative ENT genes (TcrNB1, TcrNB2, TcrNT1 and TcrNT2) were cloned and expressed in genetically adapted Trypanosoma brucei procyclic cells from which… CONTINUE READING
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