Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.

@article{Mutzel1987CloningAC,
  title={Cloning and cDNA sequence of the regulatory subunit of cAMP-dependent protein kinase from Dictyostelium discoideum.},
  author={Rupert Mutzel and M L Lacombe and Martha N. Simon and Jean de Gunzburg and M. Veron},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 1},
  pages={6-10}
}
cDNA clones encoding the regulatory subunit of the cAMP-dependent protein kinase (ATP:protein phosphotransferase, EC 2.7.1.37) from Dictyostelium discoideum were isolated by immunoscreening of a cDNA library constructed in the expression vector lambda gt11. High-affinity cAMP-binding activity was detected in extracts from bacteria lysogenized with these clones. Nucleotide sequence analysis of three overlapping clones allowed the determination of a 1195-base-pair cDNA sequence coding for the… CONTINUE READING