Cloning and bioinformatic analysis of an acidophilic β-mannanase gene, Anman5A, from Aspergillus niger LW-1


Using 3′ and 5′ rapid amplification of cDNA ends (RACE) techniques, the full-length cDNA sequence of the Anman5A, a gene that encodes an acidophilic β-mannanase of Aspergillus niger LW-1 (abbreviated to AnMan5A), was identified from the total RNA. The cDNA sequence was 1417 bp in length, harboring 5′- and 3′-untranslated regions, as well as an open reading… (More)
DOI: 10.1134/S000368381205016X


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