Cloning and analyzing 5′ flanking sequences of a tissue-specifically expressed gene derived from mouse nasopharynx

@article{Zhang2001CloningAA,
  title={Cloning and analyzing 5′ flanking sequences of a tissue-specifically expressed gene derived from mouse nasopharynx},
  author={Ling Zhang and Kaitai Yao and Ke Lan and Hong Li and Xiangling Feng and Zhiwei He and Weinong Han},
  journal={Nature Genetics},
  year={2001},
  volume={27 Suppl 1},
  pages={68-68}
}
s: Session II 6 8 We have used a two-dimensional restriction landmark genomic scanning technique to identify gene amplification events in esophageal adenocarcinomas. We cloned a multicopy DNA fragment from a tumor two-dimensional gel and confirmed genomic amplification encompassing this fragment by Southern blot analysis. The corresponding DNA sequence was searched by BLAST, which allowed the use of an electronic polymerase chain reaction to map the amplicon to 19q12. We then characterized the… CONTINUE READING