Cloning and analysis of translational control for genes encoding the Cfr9I restriction-modification system.

@article{Lubys1994CloningAA,
  title={Cloning and analysis of translational control for genes encoding the Cfr9I restriction-modification system.},
  author={Arvydas Lubys and S Menkevicius and Albertas Timinskas and Viktoras Butkus and A. Janulaitis},
  journal={Gene},
  year={1994},
  volume={141 1},
  pages={
          85-9
        }
}
The complete type-II Cfr9I restriction-modification (R-M) system of Citrobacter freundii strain RFL9, recognizing the DNA sequence CCCGGG, has been cloned and expressed, and functionally active enzymes have been produced in Escherichia coli. Both the methyltransferase (MTase; M.Cfr9I) and restriction endonuclease (ENase; R.Cfr9I) were found to be encoded on a 2.3-kb cloned fragment in the same transcriptional orientation, but differing in translational phases. The last codon (underlined) (ATGA… CONTINUE READING
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