Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe.

@article{Shimoda1987CloningAA,
  title={Cloning and analysis of transcription of the mei2 gene responsible for initiation of meiosis in the fission yeast Schizosaccharomyces pombe.},
  author={Chikashi Shimoda and Masahiro Uehira and Masao Kishida and Hitoshi Fujioka and Yuichi Iino and Yoshio Watanabe and Mami Yamamoto},
  journal={Journal of bacteriology},
  year={1987},
  volume={169 1},
  pages={93-6}
}
We have isolated a hybrid plasmid, pDB(mei2)2, containing a 7.4-kilobases (kb) DNA fragment from a Schizosaccharomyces pombe genomic library which is able to complement the mei2 mutation of S. pombe. Integration of the cloned DNA sequence at the mei2 site on chromosome I demonstrated that it contained the mei2 gene. This gene was localized on a 4.7-kb HindIII-PvuII fragment in the subclone pFMV402. Transcriptional regulation was studied by Northern blot analysis in which polyadenylated RNA was… CONTINUE READING

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