Cloning and analysis of the simocyclinone biosynthetic gene cluster of Streptomyces antibioticus Tü 6040

@article{Galm2002CloningAA,
  title={Cloning and analysis of the simocyclinone biosynthetic gene cluster of Streptomyces antibioticus T{\"u} 6040},
  author={Ute Galm and Judith Schimana and H. -P. Fiedler and J{\"u}rgen E. Schmidt and Shu-Ming Li and Lutz Heide},
  journal={Archives of Microbiology},
  year={2002},
  volume={178},
  pages={102-114}
}
The biosynthetic gene cluster of the aminocoumarin antibiotic simocyclinone D8 was cloned by screening a cosmid library of Streptomyces antibioticus Tü 6040 with a heterologous probe from a gene encoding a cytochrome P450 enzyme involved in the biosynthesis of the aminocoumarin antibiotic novobiocin. Sequence analysis of a 39.4-kb region revealed the presence of 38 ORFs. Six of the identified ORFs showed striking similarity to genes from the biosynthetic gene clusters of the aminocoumarin… CONTINUE READING