Cloning and analysis of the replication origin and the telomeres of the large linear plasmid pSLA2-L in Streptomyces rochei

@article{Hiratsu2000CloningAA,
  title={Cloning and analysis of the replication origin and the telomeres of the large linear plasmid pSLA2-L in Streptomyces rochei},
  author={Keiichiro Hiratsu and Susumu Mochizuki and Haruyasu Kinashi},
  journal={Molecular and General Genetics MGG},
  year={2000},
  volume={263},
  pages={1015-1021}
}
The replication origin and both terminal segments were cloned from the large linear plasmid pSLA2-L in Streptomyces rochei 7434AN4. The basic replicon consists of a 1.9-kb DNA fragment, which contains the genetic information required for autonomous replication in circular form. Sequence analysis revealed two ORFs, RepL1 and RepL2, with no similarity to any of the replication initiator proteins in the database. Deletion and mutational analysis showed that RepL1 is essential for replication and… CONTINUE READING

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