Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus.

Abstract

The genomic region encoding the type IIS restriction-modification (R-M) system HphI (enzymes recognizing the asymmetric sequence 5'-GGTGA-3'/5'-TCACC-3') from Haemophilus parahaemolyticus were cloned into Escherichia coli and sequenced. Sequence analysis of the R-M HphI system revealed three adjacent genes aligned in the same orientation: a cytosine 5… (More)

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@article{Lubys1996CloningAA, title={Cloning and analysis of the genes encoding the type IIS restriction-modification system HphI from Haemophilus parahaemolyticus.}, author={Arvydas Lubys and Judita Lubiene and Saulius Kulakauskas and K Stankevicius and Albertas Timinskas and A. Janulaitis}, journal={Nucleic acids research}, year={1996}, volume={24 14}, pages={2760-6} }