Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.

@article{Yip1994CloningAA,
  title={Cloning and analysis of the Saccharomyces cerevisiae MNN9 and MNN1 genes required for complex glycosylation of secreted proteins.},
  author={C L Yip and S K Welch and Franz Klebl and Teresa L. Gilbert and P Seidel and F J Grant and P J O'Hara and Vivian L. Mackay},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1994},
  volume={91 7},
  pages={
          2723-7
        }
}
Proteins secreted by the yeast Saccharomyces cerevisiae are usually modified by the addition at asparagine-linked glycosylation sites of large heterogeneous mannan units that are highly immunogenic. Secreted proteins from mnn1 mnn9 mutant strains, in contrast, have homogeneous Man10GlcNAc2 oligosaccharides that lack the immunogenic alpha 1,3-mannose linkages. We have cloned and sequenced the MNN9 and MNN1 genes, both of which encode proteins with the characteristics of type II membrane proteins… CONTINUE READING

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