Cloning and analysis of the DNA polymerase-encoding gene from Thermus caldophilus GK24.

Abstract

The gene encoding Thermus caldophilus GK24 (Tca) DNA polymerase was cloned into Escherichia coli using the structural gene coding for Thermus aquaticus YT-1 (Taq) DNA polymerase as a hybridization probe. The nucleotide sequence of the cloned DNA was determined. The primary structure of the Tca DNA polymerase was deduced from the nucleotide sequence. The Tca… (More)

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@article{Kwon1997CloningAA, title={Cloning and analysis of the DNA polymerase-encoding gene from Thermus caldophilus GK24.}, author={S. T. Kwon and J. S. Kim and J. H. Park and H. K. Kim and D. S. Lee}, journal={Molecules and cells}, year={1997}, volume={7 2}, pages={264-71} }