Cloning and analysis of a cDNA encoding a putative serine protease comprising two trypsin-like domains of Trichinella spiralis

@article{Trap2005CloningAA,
  title={Cloning and analysis of a cDNA encoding a putative serine protease comprising two trypsin-like domains of Trichinella spiralis},
  author={Catherine Trap and Baoquan Fu and Franck Le Guerhier and Mingyuan Liu and Danielle Le Rhun and Thibault Romand and Catherine Perret and Radu Blaga and Pascal Boireau},
  journal={Parasitology Research},
  year={2005},
  volume={98},
  pages={288-294}
}
The cDNA encoding a putative serine protease, TsSerP, was cloned by degenerative polymerase chain reaction and screening of the cDNA library from Trichinella spiralis adult–newborn larvae stage. Sequence analysis revealed the presence of two trypsin-like serine protease domains flanking a hydrophilic domain, with the catalytic triad residue histidine in the alpha domain substituted by an arginine residue. Southern blots indicated that this was a single copy gene in the parasite genome. Northern… CONTINUE READING