Cloning and analysis of MART-1/Melan-A human melanoma antigen promoter regions.

@article{Butterfield1997CloningAA,
  title={Cloning and analysis of MART-1/Melan-A human melanoma antigen promoter regions.},
  author={Lisa H Butterfield and Thomas Stoll and Rachel Lau and James Steven Economou},
  journal={Gene},
  year={1997},
  volume={191 2},
  pages={129-34}
}
The MART-1/Melan-A human melanoma tumor antigen can be recognized by T lymphocytes and appears to be involved in tumor regression. To study the transcriptional regulation of this important gene, the 5' untranslated (UT) region of the MART-1/Melan-A gene was cloned and sequenced. Human melanoma cell lines were screened for MART-1/Melan-A mRNA expression. Primer extension and northern analysis were performed to confirm the mRNA size and start site. Several overlapping fragments of 5'UT were… CONTINUE READING