Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase from Streptomyces cacaoi.

@article{Lenzini1987CloningAA,
  title={Cloning and amplified expression in Streptomyces lividans of the gene encoding the extracellular beta-lactamase from Streptomyces cacaoi.},
  author={Mauro V. Lenzini and Satoshi Nojima and J. A. Maquet Dusart and Hiroshi Ogawara and Philippe Dehottay and Jean Marie Fr{\`e}re and Jean M. Ghuysen},
  journal={Journal of general microbiology},
  year={1987},
  volume={133 10},
  pages={
          2915-20
        }
}
A 19 kb SphI DNA fragment containing the gene for the extracellular active-site serine beta-lactamase of Streptomyces cacaoi KCC-SO352 was cloned in Streptomyces lividans TK24 using the high-copy-number plasmid pIJ702 as vector. A 30-fold higher yield of beta-lactamase was obtained from S. lividans strain ML1, carrying the recombinant plasmid pDML51, than from S. cacaoi grown under optimal production conditions. In all respects (molecular mass, isoelectric point, kinetics of inhibition by beta… CONTINUE READING

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Induction of a Streptomyces cacaoi β-lactamase gene cloned in S. lividans

  • Molecular and General Genetics MGG
  • 1992
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3-Lactamase Expression inStreptomyces cacaoi

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CITES BACKGROUND & METHODS
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BlaB, a protein involved in the regulation of Streptomyces cacaoi β-lactamases, is a penicillin-binding protein

C. Raskin, C. Gérard, +4 authors J. Dusart
  • Cellular and Molecular Life Sciences CMLS
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Genetics and Product Formation in Streptomyces

  • Federation of European Microbiological Societies Symposium Series
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