Corpus ID: 88052023

Cloning and Prokaryotic Expression of Interferon Gamma Full Gene in Pig

@inproceedings{Chaoying2012CloningAP,
  title={Cloning and Prokaryotic Expression of Interferon Gamma Full Gene in Pig},
  author={Ma Chao-ying},
  year={2012}
}
To amplify pig interferon-γ(pIFN-γ) cDNA from ConA-activated peripheral-blood lymphocyte by RT-PCR,then was cloned into pMD18-T and sequenced,and recombined with pET32a(+) vector,the target protein His×6 IFN-γ was induced by IPTG;the sequence analysis showed that the nucleotide sequences of the IFN-γ in the study shared 98.6% to 100.0% homology with that of pig published in GenBank,and the identity of amino acid was 96.4% to 99.4%.This recombinant protein,a molecular weight of about 27 ku and… Expand