Cloning and Nucleotide Sequence of r-Polyglutamate Production Stimulating Factor on Bacillus subtilis (natto) Plasmid, pUH1

Abstract

The gene coding for y-polyglutamate production stimulating factor (psf) from Bacillus subtilis (n&o) plasmid pUH1 was cloned and sequenced in Bacillus subtilis host. T h e activity of y-glutamyltranspeptidase (r-GTP) in B. subtilis host, which was introduced the psf gene was relatively high level, while its activity in E. coli host could not be detected… (More)

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