Cloning and Molecular Analysis of Extracellular Lipase Gene From Native and Mutant Yarrowia lipolytica Strains

@inproceedings{Darvishi2013CloningAM,
  title={Cloning and Molecular Analysis of Extracellular Lipase Gene From Native and Mutant Yarrowia lipolytica Strains},
  author={Farshad Darvishi},
  year={2013}
}
biotechnology. Objectives: The aims of the study were cloning and molecular analysis of extracellular lipase genes from DSM3286 native and U6 mutant strains of Y. lipolytica in Escherichia coli. Materials and Methods: LIP2 gene of Y. lipolytica DSM3286 and its mutant Y. lipolytica U6 were isolated and cloned in E. coli using the appropriate vector. The native and mutant extracellular lipase ORFs were sequenced for further analyses and finding the differences in the nucleotide sequences. Results… CONTINUE READING