Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster

  title={Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster},
  author={Oksana Bilyk and Olga N. Sekurova and Sergey B Zotchev and Andriy Luzhetskyy},
  booktitle={PloS one},
Transformation-associated recombination (TAR) in yeast is a rapid and inexpensive method for cloning and assembly of large DNA fragments, which relies on natural homologous recombination. Two vectors, based on p15a and F-factor replicons that can be maintained in yeast, E. coli and streptomycetes have been constructed. These vectors have been successfully employed for assembly of the grecocycline biosynthetic gene cluster from Streptomyces sp. Acta 1362. Fragments of the cluster were obtained… CONTINUE READING
Highly Cited
This paper has 172 citations. REVIEW CITATIONS
Recent Discussions
This paper has been referenced on Twitter 3 times over the past 90 days. VIEW TWEETS

From This Paper

Topics from this paper.


Publications citing this paper.
Showing 1-9 of 9 extracted citations

173 Citations

Citations per Year
Semantic Scholar estimates that this publication has 173 citations based on the available data.

See our FAQ for additional information.


Publications referenced by this paper.
Showing 1-10 of 52 references

Antibiotics: Current Innovations and Future Trends: Caister

  • S Sánchez, AL Demain
  • 2015
1 Excerpt

Similar Papers

Loading similar papers…