Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster

@inproceedings{Bilyk2016CloningAH,
  title={Cloning and Heterologous Expression of the Grecocycline Biosynthetic Gene Cluster},
  author={Oksana Bilyk and Olga N. Sekurova and Sergey B Zotchev and Andriy Luzhetskyy},
  booktitle={PloS one},
  year={2016}
}
Transformation-associated recombination (TAR) in yeast is a rapid and inexpensive method for cloning and assembly of large DNA fragments, which relies on natural homologous recombination. Two vectors, based on p15a and F-factor replicons that can be maintained in yeast, E. coli and streptomycetes have been constructed. These vectors have been successfully employed for assembly of the grecocycline biosynthetic gene cluster from Streptomyces sp. Acta 1362. Fragments of the cluster were obtained… CONTINUE READING
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