Cloning and Expression Analysis of Dihydroflavonol 4-Reductase Gene in Brassica juncea

@inproceedings{Yan2008CloningAE,
  title={Cloning and Expression Analysis of Dihydroflavonol 4-Reductase Gene in Brassica juncea},
  author={Mingli Yan and Xianjun J. Liu and Zhongsong Liu and Chunyun Guan and Mouzhi Yuan and Xing-hua Xiong},
  year={2008}
}
Abstract Dihydroflavonol 4-reductase ( DFR ) gene is a key gene of proanthocyanidins biosynthesis pathway in seed coat of Arabidopsis thaliana . The mutation of this gene brings about a transparent testa. To study molecular mechanism of seed coat color in Brassica, DFR gene was cloned from B. juncea using homology-based clone strategy. The cloned gene 1,612 bp in length contains 5 introns. The complementary DNA (cDNA) consists of 1,214 bp and has a 1,158 bp open reading frame encoding a deduced… CONTINUE READING