Cloning and Expression Analysis of Alpha-Tubulin Genes in Water Foxtail (Alopecurus aequalis)

  title={Cloning and Expression Analysis of Alpha-Tubulin Genes in Water Foxtail (Alopecurus aequalis)},
  author={Saima Hashim and Mayumi Hachinohe and Hiroshi Matsumoto},
Abstract Tubulins are encoded by small gene families in plants. Here we report the cloning and characterization of water foxtail α-tubulin genes (AaTUA), an economically important weed. The genome of water foxtail contains TUA family consisting of at least four genes. Using degenerate primers, four partial TUA genes were isolated from the complementary DNA (cDNA) of water foxtail. Using the partial gene sequences, specific primers for each TUA gene were designed and full-length TUA cDNAs were… CONTINUE READING


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