Cloning and Characterization of a Bacterial Cell-Bound Type B Carboxylesterase from Bacillus sp. BP-7

Abstract

A clone producing halos on tributyrin plates was isolated from a genomic library of Bacillus sp. BP-7. The insert contained an open reading frame that coded for a protein of 487 amino acids with homology to carboxylesterases. The cloned enzyme showed clear preference for esters of short-chain fatty acids, being classified as an esterase. Maximum activity… (More)
DOI: 10.1007/s002840110210

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