Cloning and Characterization of Two Novel Crystal Protein Genes from a Bacillus thuringiensis serovar dakota Strain

@article{Kim2003CloningAC,
  title={Cloning and Characterization of Two Novel Crystal Protein Genes from a Bacillus thuringiensis serovar dakota Strain},
  author={Ho San Kim and Hiroyuki Saitoh and Satoko Yamashita and Tetsuyuki Akao and Yu Shin Park and Minoru Maeda and Rie Tanaka and Eiichi Mizuki and Michio Ohba},
  journal={Current Microbiology},
  year={2003},
  volume={46},
  pages={0033-0038}
}
Two genes encoding the 32- and 40-kDa polypeptides of Bacillus thuringiensis strain 90-F-45-14 crystals were cloned, expressed in an acrystalliferous B. thuringiensis strain, and sequenced. The polypeptides had deduced molecular weights of 30,319 and 33,885, respectively. The amino acid sequence of the 32-kDa protein was 37.7% identical to the known sequence of a non-insecticidal parasporal protein in B. thuringiensis serovar thompsoni crystals. The sequence of the cloned 40-kDa protein was 37… CONTINUE READING