Cloning and Characterization of Two Novel Crystal Protein Genes, cry54Aa1 and cry30Fa1, from Bacillus thuringiensis Strain BtMC28

@article{Tan2009CloningAC,
  title={Cloning and Characterization of Two Novel Crystal Protein Genes, cry54Aa1 and cry30Fa1, from Bacillus thuringiensis Strain BtMC28},
  author={Furong Tan and Jun Zhu and Jie Tang and Xueming Tang and Shiquan Wang and Aiping Zheng and Ping Li},
  journal={Current Microbiology},
  year={2009},
  volume={58},
  pages={654-659}
}
Bacillus thuringiensis strain BtMC28 was isolated from the soil sample in China. Two novel crystal protein genes were found by using the PCR-RFLP method. Moreover, the full-length sequences of two novel genes were obtained by a single oligonucleotide nested (SON)-PCR upstream and downstream strategy. Sequence analysis revealed that one gene encoded a polypeptide of 673 amino acid residues with a molecular mass of 76.3 kDa, 38% identical to Cry10Aa, and the other encoded a polypeptide of 687… CONTINUE READING

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