Cloning , Sequencing , and Characterization of a Gene Cluster Involved in EDTA Degradation from the Bacterium BNC 1

@inproceedings{Bohuslvek2001CloningS,
  title={Cloning , Sequencing , and Characterization of a Gene Cluster Involved in EDTA Degradation from the Bacterium BNC 1},
  author={Jan Bohusl{\'a}vek and Jason Payne and Yong Cheng Liu and Harvey Bolton and Luying Xun},
  year={2001}
}
EDTA is a chelating agent, widely used in many industries. Because of its ability to mobilize heavy metals and radionuclides, it can be an environmental pollutant. The EDTA monooxygenases that initiate EDTA degradation have been purified and characterized in bacterial strains BNC1 and DSM 9103. However, the genes encoding the enzymes have not been reported. The EDTA monooxygenase gene was cloned by probing a genomic library of strain BNC1 with a probe generated from the N-terminal amino acid… CONTINUE READING

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