• Corpus ID: 87310553

Cloning,expression and biological characterization of Myticin A,an antimicrobial peptide from mussel(Mytilus)

@article{Weihua2005CloningexpressionAB,
  title={Cloning,expression and biological characterization of Myticin A,an antimicrobial peptide from mussel(Mytilus)},
  author={Feng Wei-hua},
  journal={Academic Journal of Second Military Medical University},
  year={2005}
}
  • Feng Wei-hua
  • Published 2005
  • Biology
  • Academic Journal of Second Military Medical University
Objective:To express Myticin A in the methylotrophic yeast Pichia pastoris and to test the bioactivity of the recombinant peptide in vitro.Methods: Total RNA was isolated from adult mussel body fluid and mRNA was used to construct a cDNA library.Reverse transcription PCR was used to obtained Myticin A gene fragment.A recombinant plasmid of pMD18-Myticin A was constructed.The Myticin A gene was inserted into the vector pPIC9K and the product was transformed into strain GS115.The transformants… 
1 Citations
Research progress on the mollusc immunity in China.
TLDR
This review aims to present the various immunologically significant pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), lectins, lipopolysaccharide and β-1, 3-glucan binding protein (LGBP), scavenger receptors (SRs) employed by mollucans as well as highlighting immune proteolytic cascade, TLR signaling pathway and an extensive repertoire of immune effectors.