Cloning, yeast expression, isolation, and vaccine testing of recombinant Ancylostoma-secreted protein (ASP)-1 and ASP-2 from Ancylostoma ceylanicum.

@article{Goud2004CloningYE,
  title={Cloning, yeast expression, isolation, and vaccine testing of recombinant Ancylostoma-secreted protein (ASP)-1 and ASP-2 from Ancylostoma ceylanicum.},
  author={G. Goud and B. Zhan and Kashinath Ghosh and A. Loukas and J. Hawdon and A. Dobardzic and Vehid Deumic and S. Liu and R. Dobardzic and B. Zook and Q. Jin and Y. Liu and Laura Hoffman and Sophia Chung-Debose and R. Patel and S. M{\'e}ndez and P. Hotez},
  journal={The Journal of infectious diseases},
  year={2004},
  volume={189 5},
  pages={
          919-29
        }
}
  • G. Goud, B. Zhan, +14 authors P. Hotez
  • Published 2004
  • Biology, Medicine
  • The Journal of infectious diseases
  • cDNAs encoding 2 Ancylostoma-secreted proteins (ASPs), Ancylostoma ceylanicum (Ay)-ASP-1 and Ay-ASP-2, were cloned from infective third-stage larvae (L3) of the hookworm A. ceylanicum and were expressed as soluble recombinant fusion proteins secreted by the yeast Pichia pastoris. The recombinant fusion proteins were purified, adjuvant formulated, and injected intramuscularly into hamsters. Hamsters vaccinated either by oral vaccination with irradiated L3 (irL3) or by injections of the adjuvants… CONTINUE READING
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