Cloning, sequencing and expression of the Taq I restriction-modification system.

@article{Slatko1987CloningSA,
  title={Cloning, sequencing and expression of the Taq I restriction-modification system.},
  author={Barton E. Slatko and Jack S. Benner and T Jager-Quinton and Larry Moran and T G Simcox and Elisabeth M Van Cott and Geoffrey G. Wilson},
  journal={Nucleic acids research},
  year={1987},
  volume={15 23},
  pages={9781-96}
}
The Taq I modification and restriction genes (recognition sequence TCGA) have been cloned in E. coli and their DNA sequences have been determined. Both proteins were characterized and the N-terminal sequence of the endonuclease was determined. The genes have the same transcriptional orientation with the methylase gene 5' to the endonuclease gene. The methylase gene is 1089 bp in length (363 amino acids, 40,576 daltons); the endonuclease gene is 702 bp in length (234 amino acids, 27,523 daltons… CONTINUE READING