Cloning, sequencing and expression of the S protein gene from two geographically distinct strains of canine coronavirus.

@article{Horsburgh1995CloningSA,
  title={Cloning, sequencing and expression of the S protein gene from two geographically distinct strains of canine coronavirus.},
  author={Brian C Horsburgh and T. D. Brown},
  journal={Virus research},
  year={1995},
  volume={39 1},
  pages={63-74}
}
The gene encoding the spike (S) protein from two geographically distinct strains (American and British) of canine coronavirus (CCV) was cloned and sequenced. The nucleotide sequence revealed open reading frames of 1443 or 1453 amino acids, respectively. Structural features include an N-terminal hydrophobic signal sequence, a hydrophilic cysteine-rich cluster near the C-terminus, two heptad repeats and 29 or 33 potential N-glycosylation sites. Pairwise comparisons of S amino acid sequences from… CONTINUE READING