Cloning, sequencing and analysis of the 16S-23S rDNA intergenic spacers (IGSs) of two strains of Vibrio vulnificus.

Abstract

According to the conserved sequences flanking the 3' end of the 16S and the 5' end of the 23S rDNAs, PCR primers were designed, and the 16S-23S rDNA intergenic spacers (IGSs) of two strains of Vibrio vulnificus were amplified by PCR and cloned into pGEM-T vector. Different clones were selected to be sequenced and the sequences were analyzed with BLAST and… (More)

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Cite this paper

@article{Deng2006CloningSA, title={Cloning, sequencing and analysis of the 16S-23S rDNA intergenic spacers (IGSs) of two strains of Vibrio vulnificus.}, author={Xian-Yu Deng and Xiaoyan Chen and Zhi-xue Wang and Pu Ou and Jian-guo He}, journal={Yi chuan xue bao = Acta genetica Sinica}, year={2006}, volume={33 4}, pages={365-72} }