Cloning, sequencing, and transcriptional analysis of the recA gene of Pseudomonas cepacia.

@article{Nakazawa1990CloningSA,
  title={Cloning, sequencing, and transcriptional analysis of the recA gene of Pseudomonas cepacia.},
  author={Teruko Nakazawa and Mitsuo Kimoto and Miho Abe},
  journal={Gene},
  year={1990},
  volume={94 1},
  pages={83-8}
}
A recombinant plasmid carrying the recA gene of Pseudomonas cepacia complements a recA mutation of Escherichia coli and restores UV and methylmethane sulfonate resistance, as well as recombinational proficiency. The predicted amino acid (aa) sequence of P. cepacia RecA (347 aa; Mr, 37256) is highly homologous to the RecA proteins from Thiobacillus ferrooxidans (74% aa homology), Pseudomonas aeruginosa (72%), E. coli (71%), Anabaena variabilis (61%), and Synechococcus sp. strains PCC7002 (59… CONTINUE READING